Peptide Mapping Fingerprint. A peptide map may be viewed as a fingerprint of a protein. Reversed-phase liquid chromatography RPLC with UV.
Peptide mapping is usually performed on an isolated protein or a protein mixture. 17 When a protein is digested with a protease a set of peptides unique to this protein is produced. It is obtained at the end of several chemical processes and it helps to understand the protein being analyzed.
Peptide mapping is the most commonly used identity test for proteins1.
It is obtained at the end of several chemical processes and it helps to understand the protein being analyzed. Peptide mass fingerprinting PMF also known as protein fingerprinting is an analytical technique for protein identification in which the unknown protein of interest is first cleaved into smaller peptides whose absolute masses can be accurately measured with a mass spectrometer such as MALDI-TOF or ESI-TOF. Although most peptide mapping experiments use trypsin to produce peptides other enzymes eg Lys-C Glu-C etc can be used depending on the experimental requirement. Complete cleavage of peptide bonds is more likely to occur.
