Peptide Mapping Method. Identifying a protein using peptide mapping requires digesting the protein into peptides prior to MS analysis. A peptide map of a protein provides the specificity needed for an identity test.
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The peptide mapping is a method developed to analyze the properties of proteins evaluate the identity and stability and detect the mutation. Identifying a protein using peptide mapping requires digesting the protein into peptides prior to MS analysis. Although most peptide mapping experiments use trypsin to produce peptides other enzymes eg Lys-C Glu-C etc can be used depending on the experimental requirement.
Peptide mapping is not a general method but involves developing specific maps for each unique protein.
Peptide mapping is an identity test for proteins especially those obtained by r-DNA technology. 1 It is used to help confirm the identity of a protein therapeutic and to monitor degradation such as oxidation or deamidation. Peptide mapping is a critical step during biotherapeutic characterization. It involves the chemical or enzymatic treatment of a protein resulting in the formation of peptide fragments followed by separation and identification of the resultant fragments in a reproducible manner.
