Peptide Mapping Of Insulin. Peptide mapping is not a general method but involves developing specific maps for each unique protein. Peptide mapping is a comparative procedure because the information obtained compared to a Reference Standard or Reference Material similarly treated confirms the primary structure of the protein is capable of detecting whether alterations in structure have occurred and demonstrates process consistency and genetic stability.
Hara S1 Rosenfeld R Lu HS. Human insulin even though they differ by one amino acid threonine in place of serine Reference 1. Comparisons of peptide maps of rat liver insulin-sensitive phosphodiesterase PDE with rat brain calmodulin-sensitive PDE suggest that some peptides co-migrate 31-43 co-migration.
IDE-N and IDE-C are shown in dark green and light green respectively.
A peptide map may be viewed as a fingerprint of a protein. Variations of these methods will be indicated when appropriate in specific monographs. Download the Peptide Mapping PDG Sign-Off Cover Page. Insulin 58K 8.
