Peptide Mapping Pdf. Peptide mapping is usually performed on an isolated protein or a protein mixture. Whether you require more resolution faster throughput or need to focus on a particular aspect of a protein the UPLC.
The digest is carried out under reducing conditions which provides NLT 80 digestion. Page 2 of 13 Peptide Mapping Draft December 12 2013. Peptide mapping is an identity test for proteins especially those obtained by r-DNA technology.
PEPTIDE MAPPING Peptide mapping is an identity test for proteins especially those obtained by rDNA technology.
Peptide mapping is a widely used technique for the characterization of proteins. It involves the chemical or enzymatic treatment of a protein resulting in the formation of peptide fragments followed by separation and identification of these fragments in a reproducible manner. Peptide mapping is a key part of the ICH Q6B guidelines for characterization and confirmation of biopharmaceuticals in support of new marketing applications. Although most peptide mapping experiments use trypsin to produce peptides other enzymes eg Lys-C Glu-C etc can be used depending on the experimental requirement.
