Peptide Mapping Principle. Peptide mapping involves controlled cleavage of a pure protein with small amounts of a pure protease to generate peptides of characteristic reproducible sizes. Peptide mapping is protein fingerprinting.
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The method was developed in 1993 by several groups independently. Peptide mapping is a key analytical method for studying the primary structure of proteins. For recombinant protein pharmaceuticals such as monoclonal antibodies mAbs and antibody-drug conjugates ADCs peptide mapping is used for proof of.
Peptide mapping is a frequently-performed and necessary analytical technique and its use is only likely to increase with ever-growing biopharmaceutical pipelines and regulatory guidelines.
Although most peptide mapping experiments use trypsin to produce peptides other enzymes eg Lys-C Glu-C etc can be used depending on the experimental requirement. The principle behind peptide mapping is straightforward. Mass spectrometry is an indispensable tool for peptide and protein analysis owing to its speed sensitivity and versatility. To get the highest sequence coverage possible it is important to select the right proteases.
